Species: Human Host Species: E. coli MW: 18 kDa Genbank #: NM_003347 Tag(s): N-terminal His-tag a.a: 2–end Description:UbcH7, also known as UBE2L3 (GenBank Accession No. NM_003347), a.a. 2-end, with N-terminal His-tag, MW = 18 kDa, expressed in an E. coli cell expression system. UniProt P68036 Synonym(s): UbcH7, UBE2L3, ubiquitin-conjugating enzyme E2L3 Assay Conditions: 50 µl reaction mix (4 mM ATP, 5 mM MgCl₂, 1 mM DTT, UBE1 as an E1 (0.5 µg), UbcH7, Biotin-Ubiquitin (0.5 µg) and Smurf2 as an E3(2 µg) in 50 mM Tris pH 7.4) for 30 min at 37°C, then add to streptavidin coated plates for 30 min. at 37°C. Incubate with anti-Smurf2, thenEuropium-labeled secondary antibody, and detect by time resolved fluorescence (λex=330 nm, λem=620 nm). Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole Format: Aqueous buffer solution Storage / Stability:

>6 months at -80°C.

Application(s): Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s):

1. Katsanis, N., et al. 1998. Genomics 51: 128- 131.

2. Cook, W.J. et al., 1997. Biochemistry 36 (7): 1621–1627.

Warning(s): Avoid freeze/thaw cycles Scientific Category: Ubiquitin Conjugating Enzyme (E2) Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.