Host Species: Mouse Clonality: clone BA3R Isotype: IgG2b Background:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Description:Affinity purified Monoclonal Anti-β-Tag, clone BA3R, mouse IgG2b. Recognizes native and denatured forms of β-Actin (~42kDa). Applications: Western Blot(1:1000-10000) ,Dot Blot, ELISA, Immunocytochemistry(1:500-2000), Flow cytometry (0.1-1 µg for 10(6) cells). Optimal dilutions/concentrations should be determined by the end user. Synonym(s): Antibody, Beta-Actin Tag Formulation: 1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN₃ Species Reactivity: Mouse, Rat, Rabbit, Chicken, Human Cross Reactivity: Predicted to work with: Sheep, Horse, Guinea pig, Hamster, Cow, Pig, Chimpanzee, Cynomolgus Monkey, Japanese ricefish, Orangutan Purification: Immunogen affinity purified Immunogen: Synthetic peptide corresponding to a.a. 2 - 15 (DDDIAALVVDNGSG) of Human beta Actin cytoplasmic domain Format: Aqueous buffer solution Storage / Stability: Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. Application(s): WB, DB, ELISA, ICC, FC Warning(s): Avoid freeze/thaw cycles. Scientific Category: Antibodies Product Type: Antibody Data shown is lot-specific. Contact us for specific information on other lots.