Background:PARP2 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones. Description:The PARP2 Homogeneous Assay Kit is designed to measure PARP2 activity for screening and profiling applications. The key to the PARP2 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for PARP2 reactions. First, a sample containing PARP2 enzyme is incubated with activated DNA and biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Synonym(s): poly (ADP-ribose) polymerase 2, poly(ADP-ribosyl)transferase 2, ADPRT, ADPRTL2 Supplied As: The PARP2 Homogeneous Assay Kit comes in a convenient AlphaLISA^® format, with biotinylated histone substrate, activated DNA, primary antibody, PARP assay buffer, and purified PARP2 for 384 enzyme reactions. Format: Instructions for use: See data sheet for detailed protocol. Storage / Stability:

Stable at least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s): Great for studying enzyme kinetics and HTS applications. Reference(s): Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41. Notes: AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc. Warning(s): Avoid freeze/thaw cycles Scientific Category: PARP Product Type: Assay Kit Data shown is lot-specific. Contact us for specific information on other lots.