Bpsbioscience/Set8, Active, GST-tag/51008/50 µg,BPS Bioscience,,BPS Bioscience,bpsbioscience,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Bpsbioscience/Set8, Active, GST-tag/51008/50 µg

产品编号：51008

市场价：¥6500.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细胞因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$325.00

品牌： BPS Bioscience

公司：BPS Bioscience Inc.

公司分类：

商品介绍

Species: Human

Host Species: E. coli

MW: 44 kDa

Genbank #: NM_020382

Tag(s): N-terminal GST-tag

a.a: 195–352

Background:

SETD8 or SET domain containing lysine methyltransferase 8 is a lysine methyltransferase that monomethylates p53 in human cell lines. The monomethylation by SETD8 suppresses p53-mediated transcriptional activation of highly responsive target genes, such as p21 (CDKN1A) and PUMA (BBC3) but it has little influence on weak p53 targets. Depletion of SET8 augmented the proapoptotic and checkpoint activation functions of p53, and SETD8 expression is downregulated during DNA damage. SETD8 is a Wnt signaling mediator and is recruited by LEF1/TCF4 to regulate the transcription of Wnt-activated genes, possibly through H4K20 monomethylation at the target gene promoters.

Description:

Human SET8 (GenBank Accession No. NM_020382), a.a. 195-352 with N-terminal GST-tag, MW = 44 kDa, expressed in an E. coli expression system.

UniProt

Q9NQR1

Synonym(s):

PR-Set7, SET07, SET8, SETD8

Assay Conditions:

50 µl reaction mix (50 mM Tris, pH 8.8, 1 mM EDTA, 1 mM DTT, 40 µM S-adenosylhomocysteine, and 0-6 µg SET8) is added to the wells coated with the substrate. Incubate for 2 hr. Add antibody against methylated residue of histone H4, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminsecent substrates and read luminescence.

Formulation:

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Format:

Aqueous buffer solution

Storage / Stability:

>6 months at -80°C.

Application(s):

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Reference(s):

1. Fang,J. et al., Curr. Biol. 12 (13), 1086-1099 (2002).
2. Yin,Y. et al., Cell Cycle 7 (10), 1423-1432 (2008).

Warning(s):

Avoid freeze/thaw cycles

Scientific Category:

Methyltransferase

Product Type:

Recombinant Protein

Data shown is lot-specific. Contact us for specific information on other lots.

品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型重组酶类；分子细胞生物学；重组蛋白；分析试剂盒；底物；重组细胞系

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