请使用支持JavaScript的浏览器!
主营:激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶
℡ 4000-520-616
℡ 4000-520-616
Bpsbioscience/Sirtuin Developer (2X)/50089/6 ml
产品编号:50089
市  场 价:¥4100.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$205.00
品      牌: BPS Bioscience
公      司:BPS Bioscience Inc.
公司分类:
Bpsbioscience/Sirtuin Developer (2X)/50089/6 ml
商品介绍
Description:
SIRTAssayDeveloperisanessentialreagentforsignalgenerationinsirtuinassaysperformedwithanyofBPSBioscience’sSIRTsubstrateorfluorimetricassaykits.Anappropriatefluorimetricsubstrate,comprisinganacetylatedlysinesidechain,isfirstincubatedwithasamplecontainingSIRTactivity(nuclearorcellularextract,purifiedenzyme,bead-boundimmunocomplex,etc.).Deacetylationofthesubstratesensitizesitsothat,inasecondstep,treatmentwiththeSIRTassaydeveloperproducesafluorophore.Nicotinamideisincludedasan‘inhibitorstop’forSirtuins.
SuppliedAs:
A2xconcentrate.Productistobeused(undiluted)at1:1ratiowithSIRTreaction.
Format:
Aqueousbuffersolution
Instructionsforuse:
Ina50µLreaction,assaySIRTactivityusingSIRT(enzyme,lysate,etc.),substrate,SIRTassaybuffer,andtestinhibitor.Incubatemicrotiterplateat37°Cfor30minutes.Addanequalvolume(50µL)ofSIRTAssayDevelopertoeachwell.Incubatetheplateatroomtemperaturefor15minutes.Readsampleinamicrotiter-platereADIngfluorimetercapableofexcitationatawavelengthintherangeof350-380nmanddetectionofemittedlightintherangeof440-460nm.“Blank”value(noenzymenegativecontrol)issubtractedfromallothervalues.
Storage/StABIlity:

Store,longterm,onlyasthe2xconcentrate,at-70°C.Concentrateisstableforatleast3freeze-thawcycles,butcareshouldbetakenthatbothfreezingandthawingstepsaredonequickly(e.g.liquidN2ordryice/EtOHforfreezing,rapidfingerrubbingforthawing).Frozenstorageofsmalleraliquotsisrecommended.

Application(s):
Studyofsirtuinkinetics,regulationandinhibitorsensitivity.IdealfordrugdiscoveryandHTSapplicationsandcell-basedSIRTassays.
Reference(s):
1.K.Itoetal.Proc.Natl.Acad.Sci.USA2002998921.
2.B.HeltwegandM.JungAnal.Biochem.2002302175.
3.S.Milutinovicetal.J.Biol.Chem.200227720974
Warning(s):
Avoidfreeze/thawcycles.Useproductundiluted.
ScientificCategory:
Deacetylase
ProductType:
KitReagent
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型 重组酶类;分子细胞生物学;重组蛋白;分析试剂盒;底物;重组细胞系

自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔