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℡ 4000-520-616
℡ 4000-520-616
Bpsbioscience/SMURF2, FLAG-tag/80403/20 µg
产品编号:80403
市  场 价:¥7400.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$370.00
品      牌: BPS Bioscience
公      司:BPS Bioscience Inc.
公司分类:
Bpsbioscience/SMURF2, FLAG-tag/80403/20 µg
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 72 kDa
Genbank #: NM_022739
Tag(s): N-terminal FLAG-tag
a.a: 130–end
Description:
Human SMURF2 (GenBank Accession No. NM_022739), a.a. 130-end, with N-terminal FLAG-tag, MW= 72 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q9HAU4
Synonym(s):
SMAD specific E3 ubiquitin protein ligase 2, SMURF2
Assay Conditions:
50 µl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and SMURF2 (0-2 µg) in 50 mM Tris pH 7.4) incubate for 30 min. at 37°C, then add to streptavidin coated plates and incubate for 30 min at 37°C. Add antibody against SMURF2, incubate 1 hr. Finally, incubate 1 hr. with the Europium-labeled secondary antibody. Time resolved fluorescence measured at room temperature (λexc=330 nm, λem=620 nm).
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ?g/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Narimatsu M. et al., Cell 137 (2), 295-307 (2009)
2. Fukunaga E. et al., J. Biol. Chem. 283 (51), 35660-35667 (2008).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ubiquitin E3 ligase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型 重组酶类;分子细胞生物学;重组蛋白;分析试剂盒;底物;重组细胞系

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