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主营:激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶
℡ 4000-520-616
℡ 4000-520-616
Bpsbioscience/MMP2, His-tag (HEK293-derived)/80213/20 µg
产品编号:80213
市  场 价:¥8500.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$425.00
品      牌: BPS Bioscience
公      司:BPS Bioscience Inc.
公司分类:
Bpsbioscience/MMP2, His-tag (HEK293-derived)/80213/20 µg
商品介绍
Species: Human
Host Species: HEK293
MW: 72 kDa
Genbank #: NM_004530
Tag(s): C-terminal His-tag
a.a: 30 - 660 (end)
Description:
Human matrix metallo-peptidase-2 (MMP2), also known as gelatinase A (GenBank Accession No. NM_004530), a.a. 30 - 660 (end), with C-terminal His-tag, MW=72 kDa, expressed in an HEK293 cell expression system.
UniProt
P08253
Synonym(s):
matrix metallo-peptidase-2, MMP2, gelatinase A, MMP
Specific Activity:
≥ 511 pmole/min/µg
Assay Conditions:
Activation: This is a proform enzyme and requires activation prior to testing activity. Dilute the enzyme to 100 ng/ml in a solution containing 50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05% Brij-35, and 1 mM APMA (amino-phenyl mercuric acetate). Incubate at 37ºC for 2 hours.
Assay conditions: Reaction mixture with 10 μM 390 MMP substrate 1 (Anaspec). Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc328/em393.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Van Dijk, M., et al.,Front Genet. 2012; 3:22. 2. Klotzsche-von Ameln, A., et al., Cancer Biol Ther. 2012 Feb 15; 13(4): 216 - 223.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Protease
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型 重组酶类;分子细胞生物学;重组蛋白;分析试剂盒;底物;重组细胞系

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