Bpsbioscience/Rat PDE7B, GST-tag/60075/10 µg,BPS Bioscience,,BPS Bioscience,bpsbioscience,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Bpsbioscience/Rat PDE7B, GST-tag/60075/10 µg

产品编号：60075

市场价：¥8800.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细胞因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$440.00

品牌： BPS Bioscience

公司：BPS Bioscience Inc.

公司分类：

商品介绍

Species: Rat

Host Species: Sf9 insect cells

MW: 65 kDa

Genbank #: NM_080894

Tag(s): N-terminal GST-tag

a.a: 108–446(end)

Description:

Rat PDE7B (GenBank Accession No. NM_080894), a.a. 108-446(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

UniProt

Q8VIE4

Synonym(s):

PDE7B, phosphodiesterase 7B

Specific Activity:

≥324 pmol/min/µg.

Unit Definition:

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Assay Conditions:

Assayed using BPS's PDE7B kit, Cat. # 60371. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of rPDE7B at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Formulation:

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT.

Format:

Aqueous buffer solution

Storage / Stability:

>6 months at -80°C.

Application(s):

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Reference(s):

1. Gardner, C., et al. (2000). Biochem Biophys Res Commun. May 27;272(1):186-92.
2. Reyes-Irisarri, E., et al. (2005). Neuro-science 132(4):1173-85.
3. Zhang, L., et al. (2008). Proc. Natl. Acad. Sci. USA Dec 9;105(49):19532-7.

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

PDE

Product Type:

Recombinant Protein

Data shown is lot-specific. Contact us for specific information on other lots.

品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型重组酶类；分子细胞生物学；重组蛋白；分析试剂盒；底物；重组细胞系

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