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主营:激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶
℡ 4000-520-616
℡ 4000-520-616
Bpsbioscience/PDE11A, GST-Tag (Mouse)/60064/5 µg
产品编号:60064
市  场 价:¥8900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$445.00
品      牌: BPS Bioscience
公      司:BPS Bioscience Inc.
公司分类:
Bpsbioscience/PDE11A, GST-Tag (Mouse)/60064/5 µg
商品介绍
Species: Mouse
Host Species: Sf9 insect cells
MW: 131 kDa
Genbank #: NM_001081033
Tag(s): N-terminal GST-tag
a.a: 2-933(end)
Description:
Mouse PDE11A (GenBank Accession No. NM_001081033), a.a. 2-933(end) with N-terminal GST tag, MW= 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Synonym(s):
PDE11, phosphodiesterase 11A
Specific Activity:
≥ 1,560 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE11A at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 20 mM Glutathione, 10% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Fawcett, L. et al., PNAS. 97(7), 3207-3707 (2000).

2. Horvath, A. et al., Nature Genetics. 38, 794-800 (2006).

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型 重组酶类;分子细胞生物学;重组蛋白;分析试剂盒;底物;重组细胞系

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