主营：激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶
℡ 4000-520-616
℡ 4000-520-616
商品介绍
Background:
The Quick PCR™ Cloning Kit is a simple and highly efficient method to insert any gene or DNA fragment into a vector, without time-consuming ligation steps. Multiple PCR inserts can be added in a single step. The procedure is easy: a primer set homologous to the gene of interest and the vector are used to amplify a PCR product. This purified PCR product is incubated with restriction enzyme-digested vector and Reagent A of the Quick PCR™ Cloning Kit. Following a brief (5 minute) incubation with Reagent B, the annealed vector is ready for transformation.
Description:
The BPS Bioscience Quick PCR™ cloning kit allows for the insertion of multiple DNA fragments into the desired vector in a single reaction. No ligation step is required, saving time and effort. The method is fast, simple, and efficient. Go from PCR product to transforming cells in under an hour! Simply mix the purified PCR product with the vector in a 1:1 molar ratio (Total DNA : Vector) and follow the protocol. Efficiencies of >90% are routinely obtained. Available with or without competent cells for even greater efficiency and flexibility.
Supplied As:
Components:
Reagent A
Reagent B
Buffer A
10X BSA (0.1% w/v)
Reagent A
Reagent B
Buffer A
10X BSA (0.1% w/v)
Efficiency:
Efficiencies of >90% are typical.
Format:
assay kit
Instructions for use:
See data sheet for detailed protocol.
Storage / Stability:
Kit components may be stored at -20°C or below. Avoid freeze/thaw cycles. Upon first thaw, store all reagents in single use aliquots. Stable for at least 6 months from date of receipt, when stored as directed.
Application(s):
Kit is designed for cloning any gene or DNA fragment into any vector in a quick, single-step reaction. Can be used with multiple inserts in a single reaction. Also suitable for fixing or adding point mutations, insertions, or deletions.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Mol.Biol.Reag./Cloning
Product Type:
PCR Kit
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型 重组酶类；分子细胞生物学；重组蛋白；分析试剂盒；底物；重组细胞系
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