1UnitofHRV3CProteasecleaves>95%of100μgoftargetproteinat4°Cfor16hours.
AssayConditions:
A68kDaGST-fusionprotein(C)at1mg/mlisincubatedwithHRV3CProtease(*)ataratioof(1)1:50,(2)1:100,(3)1:200,(4)1:400(w/w)inabufferof25mMTris-HCl,pH8.0,150mMNaCl,14mMβ-mercaptoethanolat4°Cfor16hours.Thecleavedproductsare42kDaand26kDa.
Formulation:
50mMTris-HCl,pH8.0,150mMNaCl,1mMEDTA,1mMTCEP,50%glycerol
Format:
Aqueousbuffersolution
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.TimmerJC,SalvesenGS.MethodsMolBiol.2011;753:243-55.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Protease
ProductType:
RecombinantProtein
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