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主营:激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶
℡ 4000-520-616
℡ 4000-520-616
Bpsbioscience/Mouse PKM2, His-tag/50296/20 µg
产品编号:50296
市  场 价:¥9600.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$480.00
品      牌: BPS Bioscience
公      司:BPS Bioscience Inc.
公司分类:
Bpsbioscience/Mouse PKM2, His-tag/50296/20 µg
商品介绍
Species:Mouse
HostSpecies:E.coli
MW:58kDa
Genbank#:NM_011099
Tag(s):N-terminalHis-tag
a.a:2–end
Background:
PKM1andPKM2aredifferentsplicingproductsofthePKMgene(exon9forPKM1andexon10forPKM2).TheM1isoform(PKM1)isexpressedinmuscleandbrain,andtheM2isoformisexpressedduringembryogenesis,inADIposetissueandpancreaticislets,andisthepredominantformfoundincancercells.
Description:
MurinePyruvatekinaseM2(PKM2),alsoknownasPKM1/M2,isoforma(GenBankAccessionNo.NM_011099),a.a.2-end,withN-terminalHis-tag,MW=58kDa,expressedinanE.coliexpressionsystem.
UniProt
P52480
Synonym(s):
pyruvatekinasemuscle,PKM2,cytosolicthyroidhormone-bindingprotein,CTHBP,thyroidhormone-bindingprotein1,THBP1,opa-interactingprotein3,OIP3
SpecificActivity:
≥11pmol/min/µg.
AssayConditions:
A50µlPKM2reactionisconductedinabuffercontaining50mMTris(pH7.4),100mMMgCl2,250mMKCl,40mMADP,100mMphosphoenolpyruvate(PEP)atroomtemperaturefor15min.ATPproductionisdetectedusingKinase-GloLuminescentKinaseAssayPlatform.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and200mMimidazole
Format:
Aqueousbuffersolution
Storage/StABIlity:

>6monthsat–80°C

Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.SpodenG.A.etal.,Exp.CellRes.315(16),2765-2774(2009).
2.ChristofkH.R.etal.,Nature452(7184),230-233(2008).
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
MetabolicEnzymes-Kinase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型 重组酶类;分子细胞生物学;重组蛋白;分析试剂盒;底物;重组细胞系

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