Bpsbioscience/BRAF, GST-tag/40065/10 µg,BPS Bioscience,,BPS Bioscience,bpsbioscience,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Bpsbioscience/BRAF, GST-tag/40065/10 µg

产品编号：40065

市场价：¥7100.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细胞因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$355.00

品牌： BPS Bioscience

公司：BPS Bioscience Inc.

公司分类：

商品介绍

Species: Human

Host Species: Sf9 insect cells

MW: 66 kDa

Genbank #: NM_004333

Tag(s): N-terminal GST-tag

a.a: 417–766 (end)

Description:

Human BRAF (b-RAF) 9GenBank Accession #NM_004333), a.a. 417-766 (end) with N-terminal GST-tag, MW = 66 kDa, expressed in Sf9 cells using a Baculovirus expression system.

UniProt

P15056

Synonym(s):

B-RAF1, BRAF, RAFB1

Specific Activity:

44 pmol/min/µg

Assay Conditions:

Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl₂, 0.1 mg/ml BSA)containing 2 μg inactive MEK1 (BPS #40075), 5 μM ATP, and 0.1 – 1.6 ng/μl BRAF for 60 minutes at room temperature. The amount of ATP transferred was measured by BPS ATP quantification reagents.

Formulation:

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Format:

Aqueous buffer solution

Storage / Stability:

>6 months at -80°C.

Application(s):

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Reference(s):

1. Sithanandam G., et al. (1990). Oncogene. 5(12): 1775–1780.
2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.

Warning(s):

Avoid freeze/thaw cycles

Scientific Category:

Kinase/Serine-Threonine

Product Type:

Recombinant Protein

Data shown is lot-specific. Contact us for specific information on other lots.

品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型重组酶类；分子细胞生物学；重组蛋白；分析试剂盒；底物；重组细胞系

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