TheCBX1InhibitorScreeningAssayKitisdesignedtomeasuretheinhibitionofCBX1bindingtoitssubstrate.ThekitcomesinaconvenientAlphaLISA®format,withenoughbiotinylatedpeptidesubstrate,assaybuffer,detectionbufferandpurifiedGST-taggedCBX1chromodomaintoperformatotalof384enzymereactions.ThekeytothekitisthespecificbindingoftheCBX1chromodomaintothemethylated-peptidesubstrate.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequired.First,asamplecontainingCBX1andaninhibitorofchoiceisincubatedwiththebiotinylatedsubstrateforonehour.Next,acceptorbeadsareadded,thendonorbeads,followedbyre
ADIngtheAlpha-counts.