TheKineticHDAC3AssayKitisacompleteassaysystemdesignedtomeasurehistonedeacetylase3(HDAC3)activitykineticallyforscreeningandprofilingapplications.Itcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100fluorescentHDAC3activitymeasurements.Inaddition,thekitincludespurifiedHDAC3/NCOR2enzymecomplexandapotentHDACinhibitor,SAHA,foruseasapositivecontrol.TheKineticHDAC3AssayKitisbasedonauniquefluorogenicsubstrateanddevelopercombination.Thisassaymethodeliminatesdealingwithther
ADIoactivity,extraction,andchromatographyaspectsoftraditionalassays.Usingthiskit,onlyonesimplestepforsettinguptheHDAC3reactiononamicrotiterplateisneededandthentheHDAC3activitylevelcanbemeasuredkineticallyusingafluorescencereader.