TheTCF/LEFReporterkitisdesignedformonitoringtheactivityofWnt/β-cateninsignalingpathwayintheculturedcells.Thekitcontainstransfection-readyTCF/LEFluciferasereportervector,whichisaWntpathway-responsivereporter.ThisreportercontainsafireflyluciferasegeneunderthecontrolofmultimerizedTCF/LEFresponsiveelementlocatedupstreamofaminimalpromoter.TheTCF/LEFreporterispremixedwithconstitutively-expressing
Renillaluciferasevectorthatservesasinternalcontrolfortransfectionefficiency.Thekitalsoincludesanon-induc
IBLefireflyluciferasevectorpremixedwithconstitutively-expressing
Renillaluciferasevectorasnegativecontrol.Thenon-inducibleluciferasevectorcontainsafireflyluciferasegeneunderthecontrolofaminimalpromoter,withoutanyadditionalresponseelements.Thenegativecontroliscriticaltodeterminingpathwayspecificeffectsandbackgroundluciferaseactivity.