TheSBEReporterkitisdesignedformonitoringtheactivityofTGFβ/SMADsignalingpathwayintheculturedcells.Thekitcontainstransfection-readySBEluciferasereportervector,whichisaTGFβpathway-responsivereporter.ThisreportercontainsafireflyluciferasegeneunderthecontrolofmultimerizedSBEresponsiveelementlocatedupstreamofaminimalpromoter.TheSBEreporterispremixedwithconstitutively-expressing
Renillaluciferasevectorthatservesasinternalcontrolfortransfectionefficiency.Thekitalsoincludesanon-induc
IBLefireflyluciferasevectorpremixedwithconstitutively-expressing
Renillaluciferasevectorasnegativecontrol.Thenoninducibleluciferasevectorcontainsafireflyluciferasegeneunderthecontrolofaminimalpromoter,withoutanyadditionalresponseelements.Thenegativecontroliscriticaltodeterminingpathway-specificeffectsandbackgroundluciferaseactivity.