FireflyluciferasehasbeenusedasasensitivereportertostudyawiderangeofBIOLOGicalresponses.HoweverthechangeoftheexpressionofFireflyluciferasereportercanbeduetoaglobaleffectinsteadofaspecificeffect.TheaccuracyofFireflyluciferasereportercanbeimprovedbynormalizingtoacontrolreporter,suchasRenillaluciferasereporter,inthesamesample.
TheDualLuciferase(Firefly-Renilla)AssaySystemisdesignedtobeusedforhigh-throughput,rapidquantitationofbothFireflyandRenillaluciferasesfromasinglesampleinmammaliancellculture.TheFireflyLuciferaseReagentisfirstaddedtothecellsinmediumdirectly.Thisreagentlysesthecellsandcontainsasubstrateforfireflyluciferasetoproducefireflyluciferaseluminescence.Next,theRenillaLuciferaseReagentisaddedtothesamewell.Itquenchesthefireflyluciferaseluminescenceandprovidesthesubstrateforrenillaluciferasetoproducerenillaluciferaseluminescence.Thelightproductionofbothreactionscanbeconvenientlymeasuredonaluminometer.Thisassaysystemhasseveralfeatures:
•Sensitive–highlysensitivedetectionoffireflyluciferaseactivityandRenillaluciferaseactivity.
•Stable–theluciferasesignaloutputisstableformorethanonehour,providingflexibilitywithregardtoincubationtime
•High-throughput–simplehomogeneousprotocolminimizeshandlingstepstosupporthigh-throughputscreeningapplications
•Compatibility–workswellwithavarietyofcommonmediacontaining0-10%serumandphenolred.
COMPONENTS:
•High-throughputdrugscreeningusingFirefly-Renilladualluciferasereporter.
BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型 重组酶类;分子细胞生物学;重组蛋白;分析试剂盒;底物;重组细胞系