Bpsbioscience/Anti–5–hmC polyclonal antibody (rabbit)/25203/50 µg,BPS Bioscience,,BPS Bioscience,bpsbioscience,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Bpsbioscience/Anti–5–hmC polyclonal antibody (rabbit)/25203/50 µg

产品编号：25203

市场价：¥7500.00

场地：美国(厂家直采)

产品分类：抗体类>一抗>多克隆抗体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$375.00

品牌： BPS Bioscience

公司：BPS Bioscience Inc.

公司分类：

商品介绍

HostSpecies:Rabbit

Clonality:polyclonal

Background:

5–hydroxymethylcytosine(5–hmC)resultsfromtheenzymaticconversionof5–methylcytosineinto5–hydroxymethylcytosinebytheTETfamilyofiron–dependentoxygenases.5–hmCbaseswererecentlydiscoveredinmammalianDNA,inPurkinjeneurons,ingranulecellsandembryonicstemcellswheretheyarepresentathighlevels(upto0.6%oftotalnucleotidesinPurkinjecells).Recentreportsindicatethat5–hmCisabundantinbraintissue,especiallyinareasthatareassociatedwithhighercognitivefunctions.Preliminaryresultsindicatethat5–hmCmayhaveimportantrolesdistinctfrom5–mC.Althoughitspreciserolehasstilltobeshown,earlyevidencesuggests5–hydroxymethylcytosinemayrepresentanewanduniquepathwaytodemethylateDNAinvolvingarepairmechanismconverting5–hmCtocytosine.Duetothestructuralsimilaritybetween5–mCand5–hmC,thesebasesareexperimentallyalmostindistinguishable.Themostcommonlyusedmethodologies(e.g.enzymaticapproaches,bisulfitesequencing)donotdistinguish5mcfrom5–hmC.Thedevelopmentofspecificantibodiesappearstobethemostpowerfulwaytodistinguishandspecificallyenrichfor5–mCand5–hmCsequences.

Description:

Polyclonalantibodyraisedinrabbitagainst5–hydroxymethylcytosineconjugatedtoKLH.

AssayConditions:

hMeDIPresultsobtainedwiththeantibodydirectedagainst5-hmC
hMeDIP(hydroxymethylatedDNAIP)wasperformedusingtheantibodyagainst5-hydroxymethylcytosine(Cat.No.25203).DNAfrommouseEScellswaspreparedwiththeGenDNAmoduleofthehMeDIPkitandsonicatedtoobtainDNAfragmentsof300-500bp.OneμgofshearedDNAwasspikedwithunmethylated(C)methylated(mC),andhydroxymethylated(hmC)controls.hMeDIPwasperformedwith3.5μgoftherabbit5-hmCantibodyandtheimmunoprecipitatedDNAwasanalysedbyqPCRusingprimersspecificforthe3differentcontrolsequences.Figure1showsthattherabbitpolyclonalantibodyagainst5-hmCishighlyspecificforthe5-hmCbasemodification(noIPwithnon-methylatedormethylatedCcontrolfragments).

Determinationoftheantibodytiter
Todeterminethetiter,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainst5-hmC(cat.No.25203),crudeserumandflowthrough,inantigencoatedwells.Theantigenusedwasthe5-hmCbasecoupledtoBSA.Byplottingtheabsorbanceagainsttheantibodydilution,thetiteroftheantibodywasestimatedtobe1:2,800.

Dotblotanalysisusingtheantibodydirectedagainst5-hmC
Todemonstratethespecificityoftheantibodyagainst5-hmC(cat.No.25203),aDotblotanalysiswasperformedusinghmC,mCandCcontrols.Onehundredto4ng(equivalentof5to0.2pmolofC-bases)ofthecontrolswerespottedonamembrane(AmershamHybond-N+).Theantibodywasusedatadilutionof1:1,000.Figure3showsahighspecificityoftheantibodyforthehydroxymethylatedcontrol.

Concentration:

50µg

Formulation:

PBScontaining0.05%azideand0.05%ProClin300.

SpeciesReactivity:

Human,mouse,broadrange

Purification:

Affinitypurified

Immunogen:

nucleotide

Format:

Aqueousbuffersolution

Storage/StABIlity:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):

hMeDIP(3.5µg/IP)
ELISA(1:100–1:500)
DB(1:1000)

Notes:

Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.

Warning(s):

Avoidfreeze/thawcycles

ScientificCategory:

Antibodies

ProductType:

Antibody

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型重组酶类；分子细胞生物学；重组蛋白；分析试剂盒；底物；重组细胞系

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