Bpsbioscience/Rat Interleukin 10/90175-B/20 µg,BPS Bioscience,,BPS Bioscience,bpsbioscience,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Bpsbioscience/Rat Interleukin 10/90175-B/20 µg

产品编号：90175-B

市场价：¥4100.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细胞因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$205.00

品牌： BPS Bioscience

公司：BPS Bioscience Inc.

公司分类：

商品介绍

Species: Rat

Host Species: E. coli

MW: 19 kDa

Genbank #: P29456

a.a: 19–178

Background:

IL-10 is produced by mouse Th2-cells following their stimulation by lectins. The main source for B-cell derived IL-10 in mice are Ly1 B-cells that express CD5 (Ly1) and CD11. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones after both antigen-specific and polyclonal activation, by B- cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. IL-10 is a homodimeric protein with subunits having a length of 160 amino acids. Human Interleukin-10 shows 73% amino acid homology with mouse Interleukin-10. The murine IL-10 receptor has been cloned. This receptor is a protein of approximately 110 kDa that binds murine IL-10 specifically. This receptor is structurally related to receptors for IFN. The CD nomenclature for this receptor is CDw210. IL-10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL-2 and TNF-beta in Th1 T-helper subpopulations of T-cells but not of Th2 T-helper cells. This activity is antagonized by IL-4. The inhibitory effect on IFN- gamma production is indirect and appears to be the result of a suppression of IL-12 synthesis by accessory cells. In the human system, IL-10 is produced by, and down-regulates the function of Th1 and Th2 cells.

Description:

Recombinant Rat Interleukin-10 is a disulfide-linked monomer protein consisting of 161 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding rat Interleukin-10 mature chain was expressed in E. coli.

UniProt

P29456

Synonym(s):

IL-10, Interleukin 10, Cytokine Synthesis Inhibitory Factor, CSIF, TGIF, IL10

Purity:

≥95% by SDS-PAGE and HPLC

Endotoxin Level:

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Biological Activity:

The ED₅₀ was determined by the dose-dependent inhibition of antigen-specific T cell proliferation is less than 12 ng/ml.

Formulation:

Lyophilized from 0.2 µm filtered PBS, pH 7.

Format:

lyophilized protein

Reconstitution:

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Storage / Stability:

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Reference(s):

1. J. Immunol., Oct 2009, 183: 4475 - 4482.
2. J. Immunol., Oct 2009, 183: 4619 - 4627.
3. J. Exp. Med., Sep 2009, 206: 2235 - 2251.

Warning(s):

Avoid freeze/thaw cycles

Amino Acid Sequence:

SKGHSIRGDNNCTHFPVSQTHMLRELRAAFSQVKTFFQKKDQLDNILLTDSLLQDFKGYLGCQALSEMIKFYLVEVMPQAENHGPEIKEHLNSLGEKLKTLWIQLRRCHRFLPCENKSKAVEQVKNDFNKLQDKGVYKAMNEFDIFINCIEAYVTLKMKN

Scientific Category:

Cytokine/Growth Factors

Product Type:

Recombinant Protein

Data shown is lot-specific. Contact us for specific information on other lots.

品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型重组酶类；分子细胞生物学；重组蛋白；分析试剂盒；底物；重组细胞系

公司介绍

更多...

产品分类

更多...