TurboTEVProteasecontainsanenhancedformofacatalyticfragmentoftheN1aproteinofTobaccoetchvirus(TEV),acysteineproteasethatrecognizesthecleavagesiteofGlu-Asn-Leu-Tyr-Phe-Gln-GlyandcleavesbetweenGlnandGly.TurboTEVProteaseisarestrictiongradeproteasethathasarobustactivityat4°Cwithhighspecificityandgreatst
ABIlity.Itdoesnotrequireanyspecialbufferforitsactivityandcanbeusedinabuffermostsuitableforthetargetprotein.TurboTEVProteaseisa52kDaproteinwithbothGSTandHis-tagssoitcanbeeasilyremovedbyeitherNi-chelatingorGlutathione(GSH)resinalongwiththecleavedtag.Inpractice,1mg(10,000units)ofTurboTEVProteasecleaves>90%of100mgofacontroltargetproteinat4°Cin16hours.Nonon-specificcleavagehasbeenobservedunderthesameconditionwhenTurboTEVProteaseandthecontroltargetproteinwasmixedat1:10ratio.