Bpsbioscience/HSP90α, His-tag/50290/200 µg,BPS Bioscience,,BPS Bioscience,bpsbioscience,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Bpsbioscience/HSP90α, His-tag/50290/200 µg

产品编号：50290

市场价：¥7900.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细胞因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$395.00

品牌： BPS Bioscience

公司：BPS Bioscience Inc.

公司分类：

商品介绍

Species: Human

Host Species: E. coli

MW: 85.5 kDa

Genbank #: NM_005348

Tag(s): C-terminal His-tag

a.a: full length

Background:

Hsp90 (90 kDa heat shock protein) is a molecular chaperone that aids protein folding and quality control for a large number of client proteins. Functional Hsp90 operates as dimer and has intrinsic ATPase activity. The Hsp90 dimer acts in concert with other chaperones (e.g. Hsp70) and is regulated by a number of co-chaperones/accessory proteins (e.g. Hop, cdc37). Hsp90 has been shown to interact with > 100 proteins and some notable clients include kinases (e.g. Raf-1), nuclear hormone receptors (e.g. estrogen receptors), transcription factors (e.g. p53), GPCRs (e.g. CB2 receptors) and ion channels (e.g. CFTR). In humans, the Hsp90beta isoform is constitutively expressed whereas the Hsp90alpha isoforms is expressed under stress conditions. Hsp90 plays an important role in some tumor cell types by stabilising mutated oncogenic proteins.

Description:

Human Heat Shock Protein 90α (GenBank Accession No. NM_005348), full length with C-terminal His-tag, MW = 85.5 kDa, expressed in an E. coli expression system.

UniProt

P07900

Synonym(s):

HSP90a, Heat Shock Protein 90a, HSP90alpha, HSP90α

Specific Activity:

Kd = 11.8 nM.

Assay Conditions:

Assayed using the BPS Hsp90α Assay kit, Cat. # 50293. A 95 µl reaction mix (20 mM HEPES pH 7.3, 50 mM KCl, 5 mM MgCl₂, 20 mM Na₂MoO₄, 0.01% Triton X-100, 100 µg/ml bovine serum albumin, 2 mM DTT, 0-30 nM Hsp90α) + 5 µl FITC-geldanamycin was incubated for 4 hrs at 4°C. Fluorescent polarization was measured at room temperature (λexc=485 nm, λem=528 nm).

Formulation:

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol and 3 mM DTT

Format:

Aqueous buffer solution

Storage / Stability:

At least 6 months at –80°C.

Application(s):

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Reference(s):

1. Wayne N. and Bolon D.N. J Biol Chem. 2007 Nov 30;282(48):35386-95.
2. Pearl L.H., et al. Biochem J. 2008 Mar 15;410(3):439-53.

Application Reference(s):
1. The quinone methide aurin is a heat shock response inducer that causes proteotoxic stress and Noxa-dependent apoptosis in malignant melanoma cells (2015)
2. Herbimycins D-F, ansamycin analogues from Streptomyces sp. RM-7-15 (2013)

Warning(s):

Avoid freeze/thaw cycles

Scientific Category:

Heat Shock Proteins

Product Type:

Recombinant Protein

Data shown is lot-specific. Contact us for specific information on other lots.

品牌介绍

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。产品类型重组酶类；分子细胞生物学；重组蛋白；分析试剂盒；底物；重组细胞系

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