TheBRD2(
BD1+BD2)TR-FRETAssayKitisdesignedtomeasuretheinhibitionofBRD2(BD1+BD2)bindingtoitssubstrateinahomogeneous384reactionformat.ThisFRET-basedassayrequiresnotime-consumingwashingsteps,makingitespeciallysuitableforhighthroughputscreeningapplications.Theassayprocedureisstraightforwardandsimple;asamplecontainingterbium-labeleddonor,dye-labeledacceptor,BRD2,substrate,andaninhibitorisincubatedforsixtyminutes.Then,thefluorescenceintensityismeasuredusingafluorescencereader.